How to get out cat urine from mattress: Save $1,000 Today
To get out cat urine from mattress fibres, blot the liquid immediately and apply a mix of white vinegar and water to the spot. Use an enzymatic cleaner to break down the uric acid and sprinkle bicarbonate of soda to neutralise the lingering ammonia odour before vacuuming.
I woke up last Tuesday to a warm, soggy patch right where my feet usually go. My cat, Jasper, was sitting on the windowsill looking remarkably proud of himself. If you have ever dealt with that sharp, stinging smell of cat pee, you know the panic that sets in. I spent the next four hours trying to save my expensive memory foam mattress from becoming a giant litter box. Getting cat urine out of a mattress is a race against the clock because once it dries and crystallises, the smell stays forever. I have tested a few different ways to fix this, and some work much better than others.
Why regular soap does not work on cat pee
In my first attempt years ago, I just used dish soap and water. I thought if it looked clean, it was clean. I was wrong. Cat urine contains uric acid, which does not dissolve in water or regular detergents. When the weather gets humid, that acid crystals reactivate and the smell comes back. This is why you need something that actually breaks down the proteins in the urine. If you do not use the right tools, your cat will smell their own scent and keep coming back to the same spot to do their business again.
The vinegar and bicarbonate of soda method
This is my preferred home remedy because most people already have these bits in their kitchen cupboard. It is cheap and does a decent job for fresh accidents. I found that the acidity of the vinegar helps to neutralise the alkaline components of the urine.
Step 1: Blotting the excess
I grabbed a stack of paper towels and pressed down hard on the wet spot. Do not rub the stain. If you rub it, you just push the pee deeper into the mattress stuffing. I stood on the paper towels to use my body weight to soak up as much as possible. I kept doing this until the towels came up dry.
Step 2: The vinegar soak
I mixed a solution of one part white vinegar and two parts cool water. I poured this directly over the stain. You want enough to reach as deep as the urine went. I let it sit for about ten minutes. The smell of vinegar is strong, but it beats the smell of Jasper’s accidents.
Step 3: The bicarbonate of soda scrub
While the area was still damp, I heavily sprinkled bicarbonate of soda over the entire patch. It started to fizz a little bit, which is normal. I let this sit for at least eight hours. It eventually turns into a crusty paste as it pulls the moisture and odour out of the bed.
Using enzymatic cleaners for deep stains
If the urine has been there for a day or more, vinegar might not cut it. This is where I suggest buying a specific product like Simple Solution or Dr. Beckmann. These products contain live bacteria or enzymes that literally eat the uric acid. I found that saturating the area with an enzymatic cleaner and then covering it with cling film helps. The cling film stops the cleaner from evaporating too fast, giving the enzymes more time to work on the tough stains. I let it stay covered for 24 hours before letting it air dry.
How different methods compare
| Method | Best For | Effectiveness |
|---|---|---|
| Vinegar and Bicarb | Fresh stains | Medium |
| Enzymatic Cleaner | Old or deep stains | High |
| Hydrogen Peroxide | Yellowing marks | Medium |
| Steam Cleaning | General cleaning | Low (can set the smell) |
Removing yellow stains with hydrogen peroxide
Sometimes the smell goes away but a nasty yellow ring remains. I used a mix of 300ml of 3 percent hydrogen peroxide, three tablespoons of bicarbonate of soda, and a tiny drop of dish soap. I puffed this on the stain using a spray bottle. I did a small test on the side of the mattress first to make sure it did not bleach the fabric. After spraying, I let it dry and the yellow mark faded significantly. It made the mattress look almost new again, which I honestly did not expect.
Mistakes I made that you should avoid
I learned the hard way that using heat is a terrible idea. I once used a hair dryer to speed things up, but the heat actually set the proteins into the fabric permanently. The smell became a part of the mattress fibres. Also, stay away from products that contain ammonia. Cat urine smells like ammonia, so if you clean with it, your cat might think another animal has moved into their territory. This usually leads to them peeing on the bed again to reclaim it.
Frequently Asked Questions (FAQs)
Can I sleep on the mattress while it is drying?
I would not recommend it. You need the area to be completely dry to ensure the bacteria are gone. If you must use the bed, place a thick layer of dry towels and a waterproof protector over the damp spot, but it will slow down the process.
How long does it take for the smell to go away?
If you use an enzymatic cleaner, the smell might actually get worse for an hour or two as the enzymes work. Once it dries completely, usually within 24 hours, the odour should be totally gone.
What if the urine went through to the wooden bed frame?
You need to treat the wood too. I suggest wiping the wood with the same vinegar solution or an enzyme spray. Wood is porous, so if you leave it, the smell will linger in the room even if the mattress is clean.
Will bleach kill the cat urine smell?
No, please do not use bleach. Mixing bleach with the ammonia in cat pee can actually create dangerous fumes. Also, bleach is very harsh and will likely ruin the mattress cover and internal foams.
Is a mattress protector worth it for pet owners?
After my experience with Jasper, I bought a high quality waterproof protector immediately. It is much easier to throw a protector in the washing machine than it is to spend a whole afternoon scrubbing foam. It is the best investment for anyone with a cat.
Taking the time to properly clean the mess ensures your bedroom stays fresh and your cat does not turn your bed into their personal toilet. Following these steps is the best way I have found on how to get out cat urine from mattress layers effectively.

I’m Derek Sutter, and I test sleep products for Bed Sleep Advisor from my home in Wyoming Ranchettes. Over the past 9 years, I’ve reviewed 60+ mattresses, everything from budget foam to premium hybrids, tracking firmness, motion isolation, and heat retention myself. Before this, I spent six years in furniture retail, where I watched customer after customer get overwhelmed by mattress shopping. That’s what I focus on now: real durability and comfort, not spec sheets.
